################生成特征表和代表序列########
cd /home/data/ssy051/container/qiime2-2019.7_ceshi-yes
nohup docker run --rm -v /home/data/ssy051/container/qiime2-2019.7_ceshi-yes:/home \
--name=qiime2.test 8e2a626d4c8d qiime dada2 denoise-paired \
--i-demultiplexed-seqs /home/seqs/demux.qza \
--p-trim-left-f 17 --p-trim-left-r 20 \
--p-trunc-len-f 260 --p-trunc-len-r 260 \
--o-table /home/results/table.qza \
--o-representative-sequences /home/results/rep-seqs.qza \
--o-denoising-stats /home/results/denoising-stats.qza --p-n-threads 40 &
####################物种注释###############
nohup docker run --rm -v /home/data/ssy051/container/qiime2-2019.7_ceshi-yes:/home \
--name=qiime2.test 8e2a626d4c8d qiime feature-classifier classify-sklearn \
--i-classifier /home/silva-132-99-nb-341F-806R_classifier.qza\
--i-reads /home/results/rep-seqs.qza \
--o-classification /home/results/taxa/taxonomy.qza --p-n-jobs 40 &
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